assays ca 2 atpase activity assay kit elabscience  (Elabscience Biotechnology)

Journal: World Journal of Cardiology

Article Title: Mechanism of myocardial damage induced by doxorubicin via calumenin-regulated mitochondrial dynamics and the calcium–Cx43 pathway

doi: 10.4330/wjc.v17.i5.104839

Figure Lengend Snippet: The concentration of intracellular free Ca 2+ of cardiomyocytes in vivo

Article Snippet: The intracellular free Ca 2+ in rat cardiac tissues was detected with the intracellular free Ca 2+ detection kit and fluorescence labeling instrument, which were obtained from Shanghai Haohai Biological Technology Co. Ltd. Cardiac tissues were digested to a single-cell suspension, which was treated with reaction reagent and a dye solution.

Techniques: Concentration Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Taurolidine inhibits influenza virus infection and prevents influenza-induced cytokine storm, vasoconstriction and lung damage

doi: 10.1007/s00018-025-05636-6

Figure Lengend Snippet: Taurolidine inhibits intracellular calcium release by influenza virus infection. ( A , B ) The intracellular Ca 2+ concentration was measured after VSMCs cells were infected IAV/H1N1 and IBV/S9-MD, respectively. ( C ) The Ca 2+ intensity in IAV/H1N1-infected VSMCs (with or without drug treatment) was measured. ( D ) Quantitative analysis of the relative calcium intensity of each group as in C . ( E ) The Ca 2+ signaling in IAV/H1N1-infected VSMCs (with or without drug treatment) was measured. ( F ) Quantitative analysis of the relative calcium intensity of each group as in E . ( G ) Western blotting results of p-MLC and MLC in VSMCs after IAV/H1N1 infection. ( H ) Western blotting results of p-MLC and MLC in VSMCs after IBV/S9-MD infection

Article Snippet: The cell suspension and mouse serum were then harvested for Ca 2+ determination performed by a kit from Genmed Scientifics Inc. U.S.A ( GMS50097.1 v.A).

Techniques: Virus, Infection, Concentration Assay, Western Blot

Journal: Food Chemistry: X

Article Title: Trash to treasure: Potential antifreeze peptide from Litopenaeus vannamei head via ultrasound-assisted autolysis

doi: 10.1016/j.fochx.2025.102395

Figure Lengend Snippet: Total sulfhydryl content (A), surface hydrophobicity (B), Ca 2+ -ATPase activity (C), fluorescence intensity of 1st F-T (D), fluorescence intensity of 5th F-T (E) and microstructure (F, magnification of 10×) of snakehead muscle with different treatments as affected by freeze-thaw cycles. Different lowercase letters within the same freeze-thaw cycle indicate significant differences ( p < 0.05).

Article Snippet: The changes in Ca 2+ -ATPase activity of MP from surimi during freeze-thaw cycle were determined by a Ca 2+ -ATPase assay kit (Nanjing Jiancheng Biochemical Institute, Nanjing, China).

Techniques: Activity Assay, Fluorescence

Journal: ACS Measurement Science Au

Article Title: Molecular Stress Response of Mitochondria during Electrostimulation Evoking Stem Cell Differentiation Revealed by Fluorescence Imaging Combined with SERS Spectra

doi: 10.1021/acsmeasuresciau.5c00005

Figure Lengend Snippet: (a) Schematic diagram showing the regulation of the DPSC differentiation process based on IES. The fluorescence imaging (b) and bright-field images (c) of DPSCs stained with AM/PI and oil red after IES treatment with 0.8 V for 5 min at 20 s of impulse width under different days, respectively. (d) Bright-field images of DPSCs stained with alizarin red during the differentiation process by IES. All the scale bars are 100 μm. (e) Average fluorescence intensity of ALP within DPSCs after IES treatment at 0.8 V for 5 min on different days during the differentiation process. (f) Fluorescence intensity of Ca 2+ within DPSCs heat map during the differentiation process after IES. (g) Immunofluorescence imaging of dentin sialophosphoprotein (DSPP) within DPSCs stimulated with IES under different days. The cell nucleus was stained with nuclear dye of 4′,6-diamidino-2-phenylindole (DAPI) (1 μM); the scale bar is 100 μm. (h) Fluorescence intensity of DSPP within DPSCs during the cell differentiation process, calculated using the software of Image-J. *** P < 0.0001, representing the statistical significance, which was calculated under the two-tailed Student’s t -test. (i) Average length changes of DPSCs after IES treatment under different days.

Article Snippet: The ATP assay kit (BC0305) and Ca 2+ assay kit (F8840) were purchased from Solarbio.

Techniques: Fluorescence, Imaging, Staining, Immunofluorescence, Cell Differentiation, Software, Two Tailed Test

Journal: Food Chemistry: X

Article Title: Effect of protein oxidation on the quality of abalone ( Haliotis discus hannai ) during frozen storage under different packaging conditions

doi: 10.1016/j.fochx.2025.102357

Figure Lengend Snippet: Changes in carbonyl content (A), sulfhydryl content (B), surface hydrophobicity (C) and Ca 2+ -ATPase activity (D) of abalone protein during −20 °C storage under different packaging.

Article Snippet: The Ca 2+ -ATPase activity was determined by the Ca 2+ -ATPase assay kit (A070–4-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China).

Techniques: Activity Assay